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Client: Mountain Coal Company CO-0038766 SCG Project No.: 305139 <br />Site: RPE Crossing <br />Acute Toxicity Test Summary <br />Test: • 48-hour static renewal using Daphnia magna <br />• 96-hour static renewal using Fathead minnows (Pimephales <br />promelas) <br />Client: Mountain Coal Company <br />Test Procedure Followed: .EPA-821-R-02-012 <br />Sample Description: 305139 <br />Time of Sample Date of Sample Time of Sample Date of Sample <br />Collection Collection Receipt Recei t <br />1330 06-06-2005 1000 06-07-2005 <br />Dilution Water: • Moderately hard laboratory reconstituted water <br />Test Organism Source: • Daphnia magna <br />The SeaCrest Group <br />• Fathead minnows <br />The SeaCrest Group <br />Reference Toxicant: • Sodium Chloride <br />The SeaCrest Group 3 <br />