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Client: Mountain Coal Company CO-0038766 SCG Project No.: 305139 <br />Site: 12PE Crossing <br />The SeaCrest Group <br />Quality Assurance Check List -Acute Whole Effluent Toxicity Test <br />Client: Mountain Coal Company SeaCrest Sample No.:305139 <br />Species Tested: Daphnia magna and Fathead minnow <br />Start Date of Test Start Date of Test <br />(Daphnia magna) (Fathead minnow) <br />06-07-2005 06-07-2005 <br />Sample received in lab properly preserved (0-6°C)? Y <br />Sample delivered on ice or equivalent? Y <br />Test initiated within 36-hours of collection? y <br />Test protocol conforms to CDPHE guidelines (Daphnia magna)? Y <br />Test protocol conforms to CDPHE guidelines (Fathead minnow)? Y <br />Average test temp. fl°C (Daphnia magna)? Y <br />Average test temp. tl°C (Fathead minnow)? Y <br />DO level >4.Omg/1; no super-saturation (Daphnia magna)? Y <br />DO level >4.Omg/I; no super-saturation (Fathead minnow)? Y <br />Mortality in control <10%, <20% for chronic (Daphnia magna)? Y <br />Mortality in control <10%, <20% for chronic (Fathead minnow)? Y <br />Daphnia magna neonates in control Q4-hours old? Y <br />Fathead minnow larvae in control 1 to 14 days old? y <br />Appropriate reference toxicity test conducted? Y <br />Lab. Ref. Tox. test results within the confidence limits for the lab? Y <br />Position: Senior Scientist <br />z <br />The SeaCrest Group 13 <br />