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4 ~ <br />;l <br />~~.. i <br /> <br />~GS~ <br />Table 1 -Colorado Acute Test Conditions <br />ADDRESS ALL CORRESPONDENCE TO: <br />COMMERCIAL TESTING 8 ENGINEERING CO. <br />4665 PARIS STREET, SUITE &200 <br />DENVER, CO 60239 <br />7EL: (303) 3734772 <br />FAX: (303) 3734791 <br />Parameter <br />C. dubia 8 D. magna <br />Fathead Minnow <br />Test Type <br />Temperature <br />Photoperiod <br />Test Chamber Size <br />Test Sample Volume <br />Renewal Period <br />Age of Organisms <br />Number per Chamber <br />Chambers Per Concentration <br />Feeding Regime Per Chamber <br />Test Duration <br />Static Renewal <br />20 +/-1 ° C <br />16 hr light/8 hr dark <br />30 ml <br />20 ml <br />24 hrs <br />< 24 hrs <br />5 <br />4 <br />0.1 ml Algae/YTC <br />48 hrs <br />Static Renewal <br />20 +/- 1 ° C <br />16 hr light/8 hr dark <br />600 ml <br />300 ml <br />24 hrs <br />1 to 14 days <br />10 <br />4 <br />0.1 ml Brine Shrimp <br />96 hrs <br />Table 2 -Methods and Equipment <br />Characteristic Method/Equipment EPA Method <br />Alkalinity Titrimetric (APHA6, 1989) EPA;310.1 <br />Ammonia, as Nitrogen Colorimetric Analysis Method EPA 350.1 <br />Chlorine, Total Residual LaMotte STC-CI Colorimeter EPA 330.4 <br />Conductivity Oakton CON 500 Cond. Meter EPA 120.1 <br />Dissolved Oxygen Orion Model 850 DO Meter EPA 360.1 <br />Hardness Titrimetric (APHA, 1989) EPA 130.2 <br />pH Fisher Model 925 pH Meter EPA 150.1 <br />Temperature NIST7 calibrated thermometer EPA 170.1 <br />References <br />American Public Health Association. 1992. Standard Methods for the Examination of Water and <br />Wastewater, 18"' Edition. American Public Health Association, Washington, D.C. <br />Colorado Department of Health. 1998. Guidelines for Conducting Whole Effluent Toxicity Tests. Water <br />Quality Control Division, Denver, Colorado 80246-1530. <br />USEPA. 1993. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine <br />Organisms. EPA/600/4-90/027F, 4'" edition. EMSL, Cincinnati, Ohio 45268. <br />a American Public Health Association <br />7 National Institute of Standards and Technology <br />Commercial Testing & Engineering Co. Minerals Services -Corporate Office <br />1919 S. Highland Ave.. Suite 21 oB. Lombard, IL 60148 t 16301953-9300 i (6301953-9306 w~wi.sgs.com <br />MemEer a/ Ne SGS Group t5pcik6 Gdndrale de Surveillencel <br />