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SCE <br />:~ <br />Table of Contents <br />ADDRESS ALL CORRESPONDENCE TO: <br />COMMERCIAL TESTING & ENGINEERING CO. <br />4665 PARIS STREET, SUITE R-200 <br />DENVER, CO 80239 <br />TEL: (303) 373-4772 <br />' FAX: (303) 373-4791 <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplement Forms' Pg. 10 <br />Abstract <br />SGS Minerals Services Division conducted a two species acute bioassay on Westem Fuels New Horizon <br />Mine effluent discharge. This discharge has been designated as outfall 007: The purpose of this <br />bioassay was to determine if the facility complies with the acute WET portion of the Colorado State <br />Industrial General Minimal Discharge Permit. The test organisms utilized were the fathead minnow <br />(Pimephales promelas) and cladoceran (Daphnia magna). Test results indicate that the effluent was not <br />acutely toxic to fathead minnow and Daphnia magna survival. Therefore, outfall 007 is in compliance <br />with the acute WET portion of its Colorado State Discharge Permit for the fourth quarter of 2003. <br />Methods <br />,, <br />A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine, <br />ouffall 007. An effluent grab sample was collected on December 1, 2003, stored on ice, and delivered to <br />SGS Minerals Services Division on the following day. The effluent sample was received at a temperature <br />of 0.0 ° C and assigned sample tracking number 072-7434-001. The bioassay was initiated <br />approximately 24 hours after sample collection. <br />Testing was conducted in accordance with the most recent protocols. specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Daphnia magna, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water at 125-220 mg/L CaCO3. Control replicates were exposed to 100% laboratory water. <br />Statistically, the Binomial method determined the LCD s2. Significant differences in mortality were <br />analyzed using the Steel's Many-One Rank test. <br />~~ <br />'Supplemental forms include statistical analysis and chainofcustody forms <br />2 LC6p - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test <br />2 Commercial Testing & Engineering Co. I Minerals Services-Corporate Office <br />1919 S. Highland Ave.. Suite 2106, Lomhard, IL 60146 t 16301953-9300 f 16301953-9306 vnvw.sgs.com <br />FdYS MemEer °I Ne SGS Graup (SociEt6 Gfn6mle Ea Surveillance) <br />