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W ~'. T. Inc. <br />I <br />III II I IIII Ill <br />999 <br />Water & Environments! TesNne /nc. 399 West 1100 North. B Orem. Ulah 84057 /8011135-9741 Fax(801 /235-9560 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: October 5, .1999 <br />PERMITTEE NAME: Western Fuels Co. <br />SITE ID: New Horizon Mine 007 <br />OUTFACE NO: <br />TEST (AnimaUAge): Ceriodaphnia Q4 hours. <br />DATE/1'IME TEST BEGAN: 9!29/99 1:00 p.m. <br />SAMPLE (Date/Type): 9/28/99 Grab. <br />DATE/['IME TEST COMPLETED: 10/1/99 1:15 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food . . <br />Aeration <br />Dissolved Oxygen <br />Wafer Replacement <br />Temperature <br />Pboto Period <br />PH .. <br />Dilution Water <br />Receiving.Water <br />Q4 hours: ~ <br />i .. i.. <br />5 organisms/15 mL/4 replicates. <br />Fed YTC. and Algae before starting test: <br />None requited. <br />i - <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C. , <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted water (±I S% hardness of the receiving <br />water). <br />None was supplied. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely. <br />/^~ ~~ AI'„w ~_ <br />Rawlings "'-j <br />Water & Endtronmental Testing, Inc. <br />Enclosure <br />