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. -W.E. T. Inc. ~~~ ~~~~~~~~~~~~~ ~~~ <br />Wafer & Environmental Testin:P Inc 399 West 1200 Norrh. B Orem. Utah 84057 /801)235-9741 Fax/801)135-9560 <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: October 5, 1999 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine 007 OUTFACE NO: <br />TEST (An)maUAge): Fathead Minnow, 7 days. SAMPLE (Date/7ype): 9!28/99 Grab. <br />DATE/I'IME TEST 6EGAN: 9/29/99 1:20 p.m. DATElI'IME TEST COMPLETED: 10/3/99 2:30 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted efFluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animal Age.at Test Stmt <br />Number of Organisms/Dilution Volume/Replicales <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />PH . <br />Dilution Water <br />Receiving Water <br />Fathead Minnow,: 7 days: ~ <br />10 organisms/I50 mU2 replicates <br />Fed before starting test and at 48 hours, newly hatched <br />brine shrimp. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 ± 1 degrees C.... , .. <br />16 hours light 8 hours dark. <br />Measured initially and et ~4:hours: <br />Reconstituted water L15% hardness of the receiving <br />water). <br />None was supplied. <br />SUMMARY <br />V `= <br />Results: X. Pass Fail ~6~j ,,gyp <br />There was NO significant effect on survival. ~ OC.~ 1 2 u~ <br />Enclosed are data sheets and statistical reports. <br />Sincere! <br />~~~C ~~~ <br />Rawlings <br />Water & Environmmtal Testing, Inc. <br />Enclosure <br />