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Client: Mountain Coal Company CO - 0038766 SCG Project No.: 305222 <br />Site: West Elk Mine 017 <br />RESULTS <br />The data sheets for the acute tests are located in Appendix 2. Table 1 summarizes the <br />results of Daphnia magna tests with the treated and untreated effluent. All treated effluents <br />reported LCSOs within 12% of the untreated LC50 result. <br />Table 1. Daphnia magna survival and LC50 calculations for treated/untreated effluent. <br />Survival in the Fathead minnow test was 100% in the 100% effluent concentration. <br />Survival was 100% in all the remaining effluent concentrations. Control survival was 100%. <br />The sample collection showed the effects of toxicity to Daphnia magna in both the <br />treated and untreated effluent samples. The dischazge fails WET (Whole Effluent Toxicity) test <br />guidelines for this species for this sampling period. <br />No significant toxicity was measured in the Fathead minnow test. The sample passes <br />WET requirements for this species for this sampling period. <br />A series of tests were performed in conjunction with the original effluent as part of the <br />pemut requirements to confirm the effects of TDS against other possible contaminants. The tests <br />appeazed to confirm that TDS, in the form of alkalinity, is the most obvious source of toxicity in <br />the West Elk Mine 017 dischazge. <br />The SeaCrest Group 7 <br />