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^ Client: Mountain Coal Company CO - 0038766 SCG Project No.: 305222 <br />Site: West Elk Mine 017 <br />TABLE OF CONTENTS <br />ACUTE TOXICITY TEST SUMMARY <br />ABSTRACT OF RESULTS <br />3 <br />4 <br />INTRODUCTION ......................................................................................................................... 5 <br />MATERIALS AND METHODS ................................................................................................. 5 <br />SAMPLE COLLECTION ............................................................................... ................................... S <br />SOURCE AND ACCLIMATION OF TEST ORGANISMS .................................... ................................... 5 <br />TEST PROCEDURES .................................................................................... ................................... S <br />TEST PROCEDURES FOR DILUTED ACUTE TESTS ....................................... ................................... 6 <br />DATA ANALYSIS ....................................................................................... ................................... 6 <br />RESULTS <br />7 <br />DISCUSSION ................................................................................................................................ 7 <br />REFERENCES .............................................................................................................................. S <br />APPENDIX 1-CHAIN OF CUSTODY FORM ....................................................................... 9 <br />APPENDIX 2 -LAB DATA SHEETS ...................................................................................... 10 <br />APPENDIX 3 - QA/QC AND REFERENCE TOXICANT TEST CHART .........................13 <br />LIST OF TABLES <br />Table 1. Daphnia magna survival and LC50 calculations for treated/untreated effluent......? <br />The SeaCrest Gronp i~ <br />