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Table 1 -Colorado Chronic Test Conditions <br />Parameter <br />Test Type <br />Temperature <br />Photoperiod <br />Test Chamber Size <br />Test Sample Volume <br />Renewal Period <br />Age of Organisms <br />Number per Chamber <br />Chambers per Concentration <br />Feeding Regime per Chamber <br />Test Duration <br />Ceriodaphnia dubla <br />Static renewal <br />25 +/- 1 ° C <br />16 hr IighU8 hr dark <br />30 mL <br />20 mL <br />24 hrs <br />< 24 hrs <br />1 <br />10 <br />0.1 ml Algae/YTC per day <br />Until 60% of control organisms <br />have 3 broods or 8 days <br />Table 2 -Methods and Equipment <br />Characteristic <br />Alkalinity <br />Ammonia <br />Chlorine, Total Residual <br />Conductivity <br />Dissolved Oxygen <br />Hardness <br />pH <br />Temperature <br />Analytical Balance <br />References <br />Method/Equipment <br />Fathead Minnow <br />Static renewal <br />25+/-1°C <br />16 hr IighU8 hr dark <br />600 mL <br />300 mL <br />24 hrs <br />< 24 hrs <br />10 <br />a <br />0.15 ml Brine shrimp 2x/day <br />7 days <br />Titrimetric <br />CHEMetrics Colorimetery K-1510 <br />CHEMetrics Colorimetery K-2504 <br />Cole-Partner Model 1500 Cond. Meter <br />YSI Model 59 DO Meter <br />Titrimetric <br />Fisher Accumet Model 910 pH Meter <br />NISTS calibrated thermometer <br />Mettler AE 240 <br />EPA Method <br />EPA 310.1 <br />EPA 120.1 <br />EPA 360.1 <br />EPA 310.1 & 130.2 <br />EPA 150.1 <br />Methods for Chemical Analysis of Water and Waste, Environmental Protection Agency, Environmental <br />Monitoring Systems Laboratory- Cincinnati (EMSL-Cl), EPA-600(4-79-020, Revised March 1983 <br />Colorado Department of Health 1998 Guidelines for Conducting Whole Effluent Toxicity Tests Water Quality <br />Control Division, Denver, Colorado 80246-1530. <br />US EPA, October 2002, Shorf-Term Methods for Estimating the Chronic Toxicity of Effluents and <br />Receiving Waters to Freshwater Organisms, Fourth Edition, U.S. Environmental Protection Agency, <br />Office of Water, Washington, D.C. EPA-821-R-02-013 <br />s National Institute of Standards and Technology <br />