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~G~ <br />Table of Contents <br />Abstract <br />Methods <br />Results <br />Narrative & Discussion <br />Table 1 -Colo. Test Conditions <br />Page 2 Table 2 -Methods and Equipment <br />Page 2 References <br />Page 3 Discharge Monitoring Report Form <br />Page 4 Reference Toxicity Data Chart(s) <br />Page 5 Supplement Forms' <br />Page 5 <br />Page 5 <br />Pg. 6-7 <br />Pg. 8-9 <br />Pg. 10 <br />Abstract <br />SGS North America Inc. conducted a two species acute bioassay on Western Fuels New Horizon Mine <br />effluent discharge. This discharge has been designated as outfall 008. The purpose of this bioassay <br />was to determine if the facility complies with the acute WET portion of the Colorado State Industrial <br />General Minimal Discharge Permit. The test organisms utilized were the fathead minnow (Pimephales <br />promelas) and cladoceran (Ceriodaphnia dubia). Test results indicate that the efFluent was not <br />acutely toxic to fathead minnow and Ceriodaphnia dubia survival. Therefore, outfall 008 is in <br />compliance with the acute WET portion of its Colorado State Discharge Permit for the first <br />quarter of 2008. <br />Methods <br />A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine, <br />outfall 008. An effluent grab sample was collected on January 10, 2006, stored on ice, and delivered to <br />SGS North America Inc. on the next day. The effluent sample was received at a temperature of 7.0 C <br />and assigned sample tracking number 072-17476-001. The bioassay was initiated approximately 26 <br />hours after sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water at 85 mg/L CaCO3, Control replicates were exposed to 100% laboratory water. <br />Statistically, the Probit and Binomial methods determined the LC~'s2. Significant differences in mortality <br />were analyzed using the Steel's Many-One Rank test. <br />Supplemental forms include statistical analysis and chain-of-custody forms <br />z LCso - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test <br />F-526 <br />