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Western Fuels FH Acute 3/29/06 Program run on 04-03-2006 at 17:00:24 'SULTS CALCULATED USING THE BINOMINAL METHOD CONC. NUMBER NUMBER PERCENT BINOMIAL EXPOSED DEAD DEAD PROB. (PERCENT) - --------- 100 --------------- 40 -------------- 24 --------------- 60.00 -------------------------- 13.4094 75 40 16 40.00 13.4094 50 40 16 40.00 13.4094 25 40 3 7.50 LESS THAN 0.001 12.5 40 0 0.00 LESS THAN 0.001 AT A CONFIDENCE LEVEL OF 95 PERCENT, THE BINOMIAL TEST SHOWS THAT THE LC50 IS ABOVE 25 THE USEFULNESS OF ANY LC50 CALCULATED FROM THIS SET OF DATA IS QUESTIONABLE BECAUSE A CONCENTRATION-EFFECT RELATIONSHIP HAS NOT BEEN DEMONSTRATED OVER A REASONABLE RANGE (e.g. <37 TO>63 OF PERCENT DEAD AN APPROXIMATE LC50 OF 86.60254 IS OBTAINED BY NONLINEAR INTERPOLATION BETWEEN 75 AND 100 RESULTS CALCULATED USING THE MOVING AVERAGE METHOD -------- ~PAN ---------------- G ----------------- LC50 --------------- 95 PERCENT ---------------------- CONFIDENCE LIMITS 2 1.229 86.603 54.634 + INFINITY 1 1.229 86.603 0.0 +INFINITY AN LC50 CALCULATED USING THE MOVING AVERAGE METHOD MAY NOT BE A VERY GOOD ESTIMATE IF THE SPAN IS MUCH LESS THAN THE NUMBER OF CONCENTRATIONS. RESULTS CALCULATED USING THE PROBIT METHOD -------------------------------------------------------------------------------- GOODNESS OF FIT ITERATIONS G H CHI-SQUARE PROBABILITY -------------------------------------------------------------------------------- 4 0.100 1.000 3.807 0.283 SLOPE = 2.766577 95 PERCENT CONFIDENCE LIMITS = LC50 = 79.28099 95 PERCENT CONFIDENCE LIMITS = - ^_1 = 11 .43696 .i PERCENT CONFIDENCE LIMITS = 1.890445 AND 3.642709 66.37806 AND 101.2978 5.214461 AND 17.42771 COMPARE RESULTS WITH ORIGINAL DATA TO SEE IF THEY ARE REASONABLE. 1 1