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~G~ <br />Narrative and Discussion <br />PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water: <br />Receiving temperature(s): <br />CO-0034142 <br />Effluent Composite <br />03/01 /04 1600, 03/03/041230 & 03/05/041500 <br />03/02/041320 <br />Reconstituted Laboratory Water <br />15.0°C, 8.0°Cand4.0°C <br />TEST ORGANISMS <br />Fathead minnow Age: <24 hrs <br />G=riodaphnia dubia Age: <24 hrs <br />' PROCEDURE <br />Chronic bioassays as outlined in the most recent protocols specified by the Water Quality Control <br />Division of the Colorado Department of Health (1998). <br />DISCUSSION <br />' According to the Empire Corporation's discharge permit (CO-0034142), a chronic WET test is <br />failed whenever there is a statistically significant difference in lethality between the control and <br />any effluent concentration less than or equal to the Instream Waste Concentration and the ICze, <br />' which represents an estimate of the effluent concentration at which 25% of the test organisms <br />demonstrate inhibition as reflected by lethality, is at any effluent concentration less than the IWC. <br />The IWC for this facility is 11.5%. <br />By this definition, the results of this bioassay indicate that effluent from outfall 1sp is not <br />chronically toxic to fathead minnow survival or chronically toxic to Ceriodaphnia dubia survival. <br />Therefore, outfall 1sp is currently in compliance with the chronic WET portion of its Colorado <br />State Discharge Permit for the first quarter of 2004. <br />' • DATE OF REPORT <br />March 15, 2004 <br />' Re ort prep red by: Sarah Mozingo Report reviewed by: Heath Smith <br />' - ~ 3 l6-oY <br />6iomonrtorin Sue sor Date Laboratory Technician Date <br />' F-526 <br />