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~G~ <br />Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative ~ Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colorado Test Conditions Page 5 Supplement Forms' Pg. 10 <br />Abstract <br />SGS Minerals Services Division conducted a two species chronic bioassay on Empire Corporation <br />effluent discharge. This discharge has been designated as ouffall tsp. The purpose of this bioassay <br />was to determine if the facility complies with the chronic WET portion of the Colorado State Permit <br />System Authorization to Discharge. The test organisms utilized were the fathead minnow (Pimephales <br />promelas) and cladoceran (Ceriodaphnia dubia). Test results indicate that the effluent was not <br />chronically toxic to fathead minnow survival or to Ceriodaphnia dubia survival. Therefore, ouffall 1sp is in <br />compliance with the chronic WET portion of its Colorado State Discharge Permit for the first quarter of <br />2004. <br />Methods <br />A two species chronic bioassay was conducted with effluent from the Empire Corporation, ouffall tsp. <br />An effluent composite sample was collected on Mach 1, 2004, stored on ice, and delivered to SGS <br />Minerals Services Division on the next day. The effluent sample was received at a temperature of 15.0 ° <br />C and assigned sample tracking number 072-8468-001. The bioassay was initiated approximately 23.0 <br />hours after sample collection. <br />Subsequent samples were collected on March 3 and March5, 2004, and delivered to SGS on the <br />following days. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Die~ision of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 11.5%, prepared using reconstituted <br />laboratory v/ater at 130 mg/L CaCO3, Control replicates were exposed to 100% laboratory water. <br />Fathead minnow survival and growth data were analyzed for significant differences using the Steel's <br />Many-One Rank tests. Ceriodaphnia dubia survival and reproduction data were analyzed for significant <br />differences using Fisher's Exact and Steel's Many-One Rank tests, respectively. ICZS's2 were determined <br />using the Bootstrap Linear Interpolation method. <br />' Supplemental forms include statistical analysis and chain-of-custody forms <br />Z Inhibition Concentration at 25% reduction <br />F-526 <br />