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STATE OF COLORADO <br />DIVISION OF MINERALS AND GEOLOGY <br />Department of Natural Resources <br />1313 Sherman St., Room 215 <br />Denver, Colorado 80203 <br />Phone: (303) 866-3567 <br />FAX: (303) 832-8106 <br />14 January 2005 <br />Philip W Ryan <br />Colorado State University - Macromoleculaz Resources <br />C-121 Microbiology Building <br />2021 Campus Delivery <br />_ _ Fort Collins, CO 80523 - _ _ <br />Deaz Mr. Ryan, <br />COLORADO <br />DIVISION OF <br />MIN sRALS <br />GEOLOGY <br />RECLAMATION•MI NING <br />SAFET Y•SCIENCE <br />Bill Owens <br />Governor <br />Russell George <br />Executive Director <br />Ronald W. Wttany <br />Division DIfeCEGr <br />Natural Resource Trustee <br />Enclosed aze samples for MALDI analysis for moleculaz weight determination. I believe <br />that the samples contain humic acids. The reported range of moleculaz weights is 700- <br />> 100,000 Daltons. I am not sure if you can scan that entire range but, if not, I am more <br />interested in the lower ranges (500- _10,000). The samples from the Edwazds Mine <br />Portal spring and the Bowie No. 2 Mine seep appeaz to have very high concentrations of <br />humic acids (maybe even in the parts-per-thousand range). The samples from the <br />Gunnison River should have lower concentrations (where dilution is probably not <br />required). <br />If there is any other analysis you think would give us additional information, please do <br />not hesitate to call. My direct line is (303} 866-4929. My e-mail for the pdf reports is <br />jim.stazk@state.co.us. Please send the bill to Colorado Division of Minerals and <br />Geology, Attention: Shazon Elliott, 1313 Sherman Street, Room 215, Denver, CO, 80203. <br />Thank you and please contact me if you have any questions. <br />Sincerely, <br />James R. Stazk <br />Environmental Protection Specialist <br />Office of Office of Colorado <br />Mined Land Reclamation Active and InaRive Mines Geological Su <br />