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~~~ ~~~~~~~~~~~~~ ~~~ inc. ! <br />1Vn1er ,... _..... _..999_ _ ........e !na 399 Wes! 1100 North, B Orem, Ula6 84057 BO!)235-9741 FoxBO!)235-9560 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: June 16, 1999 <br />PER1VaTTEE NAME: Western Fuels Co. (New Horizon Mine) NPDFS NO: 007 OUTFACE NO: <br />TEST (Anima!/Age): Ceriodaphnia <24 hoots. SAMPLE (D~eI!ype): 6/8/99 Gtab. <br />DATE/1')ME TEST BEGAN: 6/9/99 12:25 p. m. DATE/TQV)E TEST COMPLETED: 6/11/99 11:40 am <br />TEST CONDTI'IONS <br />Ceriadaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period Survival <br />was meastrred to determine if Acute Toxicity was present in the sample. <br />Animal Age at Test Start <br />Number of Organisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Wafer Replacement <br />Temperature <br />Photo Period <br />PH <br /><24 hotus. 1 <br />5 organisms/15 mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required <br />Measured daily old/new. i <br />Renewed daily. <br />20 ± 1_dggrees C. <br />16 hours Jight 8 hours dark <br />Measured initially and at 24 hours. <br />Reconstituted water L15% hardness of the receiving <br />water). <br />None was supplied <br />Dilution Water <br />Receiving Water <br />Sam Ie Concentr <br />SUM117ARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, ~~ <br />i 1 ~'~/ ~Z ~~~~ <br />Rawlings <br />Water & Environmental Testing, Inc. <br />100%. <br />r <br />Enclosure <br />