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Mountain Coal Company CO - 0038776 SCG Project No.: 304204 <br />Site: West Elk Mine <br />The SeaCrest Group <br />Quality Assurance Check List -Acute and Chronic Whole Effluent Toxicity Test <br />Client: Mountain Coal Company <br />SeaCrest Sample No.: 304204 <br />Species Tested: Daphnia magna <br />Start Date of Test Start Date of Test <br />(Daphnia magna) (Fathead minnow) <br />8-10-04 -N/A- <br />Sample received in lab properly preserved (6°C)? N* <br />Sample delivered on ice or equivalent? Y <br />Test initiated within 36-hours of collection? Y <br />Test protocol conforms to CDPHE guidelines (Daphnia magna)? Y <br />Test protocol conforms to CDPHE guidelines (Fathead minnow)? N/A <br />Average test temp. tl°C (Daphnia magna)? Y <br />Average test temp. tl°C (Fathead minnow)? N/A <br />DO level >4.Omg/1; no super-saturation (Daphnia magna)? Y <br />DO level >4.Omg/1; no super-saturation (Fathead minnow)? N/A <br />Mortality in control <10%, <20% for chronic (Daphnia magna)? Y <br />Mortality in control <10%, <20% for chronic (Fathead minnow)? N/A <br />Daphnia magna neonates in control <24-hours old? Y <br />Fathead minnow larvae in control 1 to 14 days old? N/A <br />Appropriate reference tonicity test conducted? Y <br />Lab. Ref. Tox. test results within the confidence limits for the lab? Y <br />* Sample temperature 20.3°C upon arrival to SeaCrest Lab. Sample was packed in Blue Ice. <br /> <br />The SeaCrest Group, LLC 12 <br />