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SeaCrest Project Number: 300340 <br />COLORADO -CDPS WET TEST REPORT FORM -CHRONIC <br />PERMITTEE: Seneca Coal Company CDPS No: CO-0000221 OUTFACE: 004 <br />TYPE TEST: Routine: XX Accelerated: Test Species: Ceriodaphnia dubia IWC: 17.3% <br />TEST RESULTS: LETHALITY GROWTH REPRODUCTION <br />Conc. With Stat. <br />Difference 10D% NA 12.5%, 25°~, 50%, <br />75%, 100°h <br /> PASS PASSIFAIL FAIL <br />IC25 37.5°h NA 8.6% <br /> PASS PASS/FAIL FAIL <br />STAT. METHOD USED: Statistical Diff.: Dunnett's ANOVA ,Fisher's Exact Test IC25: ICp <br />DATE: START: 11:00 AM/PM 8 / 8100 END: 11:00 AM/PM 8 / 14 / 00 <br />DILUTIONS (% EFFLUENT) <br />Measurements Control0°~ 12.5°k 25% 50°~ 75% 100°,6 Recon <br />°,b Survival for Day 1 100 100 100 100 100 100 XX <br />°b Survival for Day 2 100 100 100 100 100 100 XX <br />°h Survival for Day 3 100 100 100 90 80 90 XX <br />°h Survival for Day 4 100 100 100 90 80 80 XX <br />°k Survival for Day 5 100 100 90 80 70 70 XX <br />°h Survival for Day 6 100 100 80 70 70 60 XX <br />Survival for Day 7 XX XX XX XX XX XX XX <br />Mean 3 Brood Total 15.2 9.7 6,0 4.1 4.9 3.6 XX <br />7 Day Mean Dry Wt )()( )p( )DC XX XX XX XX <br />pH Max/Min: 7.9/7.2 7.8!7.3 7.9!7.6 8.1/7.7 8.1/7.7 8.2!7.7 XXlXX <br />Hardness (k) mg/L: Receiving Water: NA <br />Alkalinity (k) mg/L: Receiving Water: NA <br />Total Ammonia as N (x) mg/L: Initial Effluent, <br />Total Residual Chlorine, mg/L: 100°h: <0.01 <br />Were Atl Test Conditions in Conformance with [ <br />List Deviations From Test Specifications: <br />Effluent: 2752 Recon water: 90 <br />Effluent: 142 Recon water: 42 <br /><1.0 2nd Effluent: <1.0 Final Effluent: <1.0 <br />Receiving Water Used for Dilution? N <br />)ivision Guidelines? Y <br />Laboratory: THE SEACREST GROUP <br />Analyst(s): Geoff Henderson, lSt~cey B <br />