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SeaCrest Project Number. 399097 <br />COLORADO -COPS WET TEST REPORT FORM -ACUTE <br />PERMITTEE: Peabody Coal CDPS No: CO-0000221 OUTFACE: 005 <br />TYPE TEST: Routine: XX Accelerated: Test Species: Ceriodaphnia dubia <br />TEST RESULTS: LC50: >100°f° STATISTICAL METHOD: Probit <br />Test Time S Date: Begin: 11:25, March 23, 1999 End: 12:25, March 25, 1999 <br />Dilutions (Percent Effluent) <br />Measurements Control <br />o%y 12.5°fo 25% SOYo 75% 7009'o XX <br />No. ~ Start of Test 20 20 20 20 20 20 XX <br />No. live after 24 hours 20 20 20 20 20 20 XX <br />No. live after 48 hours 19 20 19 18 20 19 XX <br />No. flue after 72 hours XX XX XX Xx Xx XX XX <br />No. Live after 96 hours %X XX XX XX XX XX XX <br />Receiving Water Used for Dilution: Yes: No: XX Total Resid. Chlorine, mg/L: 100°l°: <0.01 <br />Hardness, mglL: Receiving Water. NA Effluent: 1288 Recon. Water: 90 <br />Alkalinity, mg/L: Receiving Water. NA Effluent: 123 Recon. Water. 42 <br />pH: Initial -Control: 8.0 100%: 8.2 Final -Control: 7.1 100%: 8.3 <br />Total Ammonia as N, mglL: Initial -100%: <1.0 Final - 100%: NA <br />Were All Test Conditions In Conformance With Division Guidelines? Yes <br />IF NO, LIST DEVIATIONS FROM TEST SPECIFICATIONS: Sample arrived with supersaturated <br />dissolved oxygen content. Sample aerated prior to test setup to lower DO. <br />LABORATORY: THE SEACREST GROUP <br />COMMENTS: <br />ANALYST'S NAME(S): Robert Markovic, Felicia Remington, Kelly Carr <br />SIGNATURE/DATE: /1~'1/t~ <br />