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Mountain Coal Company CO-0038776 SeaCrest Number: 303369, 303385 <br />DISCUSSION <br />The 017 discharge site failed the whole effluent toxicity test requirements for the <br />Daphnia magna. An LC50 concentration was created which caused the test to fail. This <br />species will need to proceed to accelerated testing with the discharge to determine persistence <br />of the toxicity. <br />There was no toxicity seen in the fathead minnow test. <br />REFERENCES <br />Weber, C.I. et. al. 1993. Methods for Measuring the Acute Toxicity of Effluents and Receiving <br />Waters to Freshwater and Marine Organisms (fourth edition). Environmental Protection Agency <br />Report No. EPA/600/4-90/027F. 293 pp. <br />APHA/AWWANVEF. 1998. Standard Methods for the Examination of Water and Wastewater. <br />20th Edition. American Public Health Association. <br />Hach Company. 2002. Hach Water Analysis Handbook. 4th Edition. Hach Company, <br />Loveland, Colorado. 1260 pp. <br />The Se~Crest Group 4 <br />