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ABSTRACT <br />Commercial Testing and Engineering, Co. (CT&E) conducted a two species acute bioassay on <br />the West Fork of Sylvester Gulch effluent discharge. This discharge has been designated as <br />outfa11017. The purpose of this bioassay was to determine if the facility complies with the WET <br />portion of the Colorado State Permit System Authorization to Discharge. The test organisms <br />utilized were the fathead minnow (Pimephales promelas) and the daphnid (Daphnia magna). <br />Test results indicate that the effluent was not acutely tonic to either species. Therefore, <br />Mountain Coal Company outfall 017 effluent is in compliance with the acute WET portion of its <br />Colorado State Discharge Permit for the fourth quarter of 2000. <br />t <br />COMMERCIAL TESTING & ENGINEERING CO. <br />F-C66 Lt~ <br />Original Watermarked For Your Protection oe w. <br />