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Mountain Coal Company CO - 0038766 SCG Project No.: 304225 <br />Site: West Elk Mine <br />Acute Toxicity Test Summary <br />Test: • 48-hour static renewal using Daphnia magna <br />• 96-hour static renewal using Fathead minnows (Pimephales <br />promelas) <br />Client: Mountain Coal Company <br />Test Procedure Followed: EPA-821-R-02-012 (2002) <br />Sample Description: 304225 <br />Time of Sample Date of Sample Time of Sample Date of Sample <br />Collection Collection Receipt Receipi <br />1300 8-30-04 0939 8-31-04 <br />~~~~ ~'~~ ~~ ,. ,D~ap~,hn~ magna"~ ~ .,attiea"d,~mwno~s ~'; <br />Test Initiation Time 1330 1230 <br />Test Initiation Date 8-31-04 8-31-04 <br />Test Completion Time 1245 1300 <br />Test Completion Date 9-02-04 9-04-04 <br />Dilution Water: • Moderately hard laboratory reconstituted water <br />Test Organism Source: • Daphnia magna <br />The SeaCrest Group <br />• Fathead minnows <br />The SeaCrest Group <br />Reference Toxicant: • Sodium Chloride <br />The SeaCrest Group 3 <br />