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W.E.T. Inc. III IIIIIIIIIIIIIIII <br />Warer & Environmen[aJ Tes[inP /nc. 399 Wes[ 1100 North, B Orem, Utah 84057 (8 01123 5-9 741 Fax(807J235-9560 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphr:ia <br />DATE: June 25, 2001 <br />PERMITTEE NAME: Western Fuels Co. SITE iD: New Horizon Mine OUTFACE NO: 007 <br />TEST (Animal/Age): Ceriodaphnia <24 hours. <br />DATE/I'IME TEST BEGAN: 6/20/01 11:45 a.m. <br />SAI1iPLE (Dare/Type): 6/19/01 Grab. <br />DATE/I'IME TEST COMPLETED: 6/22/01 11:00 a.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted Effluent as specified by the permit. At the end of the test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age a[ Test Start <br />Number ofOrganisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />Dissolved Oxygen <br />Water Replacement <br />Temperature <br />Photo Period <br />pH <br />Dilution Water <br />Receiving Water <br /><24 hours. <br />5 organisms/IS mL/4 replicates. <br />Fed YTC and Algae before starting test. <br />None required. <br />Measured daily old/new. <br />Renewed daily. <br />20 + I degrees C. <br />16 hours light 8 hours dark. <br />Measured initially and at 24 hours. <br />Reconstituted lab water approx. 200 mg/L hardness. <br />None was supplied. <br />SUMMARY <br />Results: X Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, <br />,~~~,~~~ <br />Rawlings <br />ater 8c Environmental Testing, Inc. <br />Enclosure <br />