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SGS <br />Table of Contents <br />Abstract <br />M Page 2 Table 2 -Methods and Equipment Page 5 <br />ethods <br />Results Page 2 References Page 5 <br /> Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Paged Reference Toxicity Data Chart(s) Pg. 8-g <br />Table 1 -Colo. Test Conditions Page 5 Supplement Forms' pg, 10 <br />SGS Minerals Services Division conducted a two species acute bioassay on Western Fuels New Horizon <br />Mine eflluent discharge. This discharge has been designated as outfall 007. The purpose of this <br />bioassay was to determine if the facility complies with the acute WET portion of the Colorado State <br />Industrial General Minimal Discharge Permit. The test organisms utilized were the fathead minnow <br />(Pimepltales promelas) and cladoceran (Daphnia magna). Test results indicate that the effluent was not <br />acutely toxic to fathead minnow and Daphnia magna survwal. Therefore, outfall 007 is In compliance <br />with the acute WET portion of its Colorado State Discharge Permit for the third quarter of 2005. <br />A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine, <br />outfall 007. An eflluent grab sample was collected on August 31, 2005, stored on ice, and delivered to <br />SGS Minerals Services Dlwsion on the next day. The effluent sample was received at a temperature of <br />15.0" C and assigned sample tracking number 072-15509-001. The bioassay was initialed <br />approximately 25 hours after sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health 11998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Daphnia magrTa, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100°~~, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water at 115-200 mglL CaCO, Control replicates were exposed to 100°ib laboratory water. <br />Statistically, the Probit and Binomial methods determined the LCy;; s'. Significant differences in mortality <br />were analyzed using the Steel's Many-One Rank test. <br />Supplemental forms InGudo slatlsNcal analysts and chain-ot-custody forms <br />° LCyo - en estimate of the concentration lethal N> SD°/, of the organisms in fhe Ilme prescribed by the teat <br />