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Mountain Coal Company CO - 0038766 SCG Project No.: 304309 <br />Site: West Elk Mine <br />RESULTS <br />The data sheets for the acute tests aze located in Appendix 2. Table 1 summazizes the <br />results of Daphnia magna tests with the treated and untreated effluent. <br />Table 1. Daphnia magna survival and LC50 calculations for treated/untreated effluent. <br />Survival in the Fathead minnow test was 98% in the 100% effluent concentration. All <br />remaining concentrations had 100% survival. Control survival was 100%. <br />DISCUSSION <br />The sample collection showed the effects of toxicity to Daphnia magna in both the <br />treated and untreated effluent samples. The discharge fails WET (whole effluent toxicity) test <br />guidelines for this sampling period. <br />No toxicity was measured in the Fathead minnow test. The sample passes WET <br />requirements for this species. <br />The results appear to confirm that TDS (mainly in the form of high alkalinity) continues <br />to be the only source of toxicity in the sample. <br />REFERENCES <br />1. Hach Chemical Company. 2002. Hach Water Analysis Handbook. Hach Chemical <br />Company. Loveland, Colorado. 1260 pp. <br />2. APHA/AWWA/WEF. 1998. Standard Methods for the Examination of Water and <br />Wastewater. 20`s Edition. American Public Health Association. <br />3. USEPA 2002. Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters <br />to Freshwater and Marine Organisms. EPA-821-R-02-012. <br />4. Colorado Department of Public Health and Environment (CDPHE). 1998. Laboratory <br />Guidelines for Conducting Whole Effluent Toxicity Tests. Water Quality Control Division. <br />The SeaCrest Group 7 <br />