HYDRO23140

• Permit No: C1980007
• IBM Index Class Name: Hydrology
• Doc Date: 1/31/2005
• Doc Name: 4th Qtr 2004 Acute Biomonitoring Tests
• From: Mountain Coal Company
• To: DMG
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

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The SeaCrestGroup Daphnid Acute Benchsheet Form #:1o2a
Louisville, CO Effective: February 2002
Permittee: t•A T. Ca!`~.-. Site: t",(F~i E11~ f~t~r.a Lab #: 30 i{~pq
IWC%: P. CI Template#: Z Sample Date: io^'~a4 Dilution Water: f+:H
Age&SOUrce: t0~.R6Lt- #t 183.Lu:~n} __ Test Started: tOdr <1`-1- iQCO Test Ended: (O ~cs04- G4~-tp
Test Conditions: ~~°io Dr~~kTiorJ i- _AlK ltd ~
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1. Exposure Chamber
i'otaS r'a.acity: 30 ml Test Solution Volume: 15 ml
Test Solution Surface Area: _
cm` Water Depth (constant): cm
(cyclic): cm
2. Feeding Schedule
Not Fed: Fed Daily:
Fed Irregularly: X Food Used: YCT, Algae
(describe): fed 1-2 hours before test
3. Aeration
None:
Before Use: ~_minutes @ -100 bubbles/min)
4. Screened Animal Enclosures
Not Used: X Used: cm diameter
5. Condition/appearance of surviving organisms at end of test (i.e., alive but immobile, loss of
or ientation, erratic movement, etc.): ALtJ~ } Wlur;/tom

6. Comments: