HYDRO23140

• Permit No: C1980007
• IBM Index Class Name: Hydrology
• Doc Date: 1/31/2005
• Doc Name: 4th Qtr 2004 Acute Biomonitoring Tests
• From: Mountain Coal Company
• To: DMG
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

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The SeaCrest Group Daphnid Acute Benchsheet Form #: 1o2a
Louisville, CO Effective: February 2002
Permittee: W1T. (-°Qf.- Site: t~l ~v- EIIC t~lin~. Lab #: 30 ~13U9
IWC %: 1 ,~ Template #: y Sample Date: I Oa1ty-1 Dilution Waler: ('nil
Age & Source: 103,804- #s 1$3. CU rri) Test Started: IOdAU4- 1115 Tes[ Ended: to3o o4- 1100
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Initials S4 OWN S e u ent recel vln recon
CO, '- _ - hardnese /ma/Il
1. Exposure Chamber
Total Capacity: 30 m{ Test Solution Volume: 1 S mi
Test Solution Surtace Area: cm` Water Depth (constant): cm
(cyclic): cm
2. Feeding Schedule
Not Fed: Fed Daily:
Fed Irregularly: X Food Used: YCT, Algae
(describe): fed 1-2 hours before test
3. Aeration
None:
Before Use: minutes @ -100 bubbles/min)
4. Screened Animal Enclosures
Not Used: X Used: cm diameter
5. Condition/appearance of surviving organism s at end of test (i.e., alive but immobile, loss of
orientation, erratic movement, etc.: i;Ytbi31

6. Comments: