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SeaCrest Project Number: 300429 <br />COLORADO -CDPS WET TEST REPORT FORM -CHRONIC <br />PERMITTEE: Seneca Coal Company CDPS No: CO-0000221 OUTFACE: 004 <br />TYPE TEST: Routine: XX Accelerated: Test Species: Pimephales promelas IWC: 7.8°h <br />TEST RESULTS: LETHALITY GROWTH REPRODUCTION <br />Conc. With Stat. <br />Difference >100% >100% NA <br /> PASS PASS PASS/FAIL <br />IC25 >100% >100% NA <br /> PASS PASS PASS/FAIL <br />STAT. METHOD USED: Statistical Diff.: Dunnett's ANOVA IC25: ICp <br />DATE: START: 14:45 AM/PM 10 / 3 / 00 END: 15:00 AM/PM 10 / 10 / 00 <br />DILUTIONS (% EFFLUENT) <br />Measurements Control 0% 6.25°h 12.5°~ 25% 50% 100% Recon <br />°~ Survival for Day 1 100 100 100 100 100 100 XX <br />°,6 Survival for Day 2 100 100 100 100 100 100 XX <br />°~ Survival for Day 3 100 100 100 100 98 t 00 XX <br />°,6 Survival for Day 4 100 100 100 95 98 100 XX <br />%Survival for Day 5 100 100 100 93 90 100 XX <br />°~ Survival for Day 6 98 100 95 93 90 98 XX <br />%Survival for Day 7 98 98 95 93 90 95 XX <br />Mean 3 Brood Total )()( )()( )p( )p( )()( )p( )()( <br />7 Day Mean Dry Wt 0.26 0.28 0.29 0.27 0.34 0.30 XX <br />pHMax/Min: 8.1!7.4 8.1!7.6 8.1/7.5 8.1/7.6 8.1!7.7 8.2!1.7 XX/)CX <br />Hardness (x) mg/L: Receiving Water: NA Effluent: 2507 Recon water: 88 <br />Alkalinity (x) mg/L: Receiving Water: NA Effluent: 133 Recon water. 70 <br />Total Ammonia as N (~) mg/L: Initial Effluent: <1.0 2nd Effluent: <1.0 Final Effluent: <1.0 <br />Total Residual Chlorine, mg/L: 100%: <0.01 Receiving Water Used for Dilution? N <br />Were All Test Conditions in Conformance with Division Guidelines? Y <br />List Deviations From Test Specifications: Effluent aerated prior to test setup -dissolved oxygen <br />supersaturated. <br />Laboratory: THE SEACREST GROUP <br />Analyst(s): Lee Wagner, Geoff Henderson <br />Signature/Date: <br />