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Mountain Coal Company SeaCrest Number. 399033 <br />ABSTRACT OF RESULTS <br />Test Concentrations: Control, 100%, 50%, 25%, 12.5%, 1.9% <br />Number of organisms in each concentration: 20 <br />Replicates at each concentration: 4 <br />Site Identification: West Elk Mine, #013 <br /> Daphnia magna Fathead <br /> minnows <br />Test vessel size 30 ml 260 ml <br />Exposure volume: 20 ml 200 ml <br />LC50 concentration: >100% >100% <br />Temperature range (°C): 19.1-20.9 19.0-21.0 <br />Dissolved oxygen range (mUl): 7.2-8.4 4.7-7.3 <br />pH range during test: 7.4-8.9 7.5-8.8 <br /> Control 100% <br />Alkalinity (mg/I as CaCO,): 40/65 2021 <br />Hardness (mgll as CaCO,): 89/89 48 <br />Total residual chlorine (mg/I): <0.01 <br />Total ammonia in effluent (mg/I): <1.0 <br />"Not analyzed. No chlorine or ammonia used in reconstituted water formulation. <br />The SeaCrest Group l3 <br />