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COLORADO DEPARTMENT OF HEALTH, Water Quality Control Division <br />Rationale - Page 8. Permit No. CO-0040827. <br />VI. TERMS AND CONDITIONS OF PERMIT <br />a) Purpose of WET Testing: (Cont.) effects. WET testing will <br />provide a more comprehensive means of evaluating the toxicity ~~E <br />a discharge than could otherwise currently be accomplished. <br />b) Instream Waste Concentration (IWC): As a condition of the <br />permit, the permittee will be required to conduct routine ' <br />monitoring For acute toxicity. Acute toxicity occurs when there <br />is a statistically significant difference in the mortality <br />observed, for Ceriodaphnia sp. (water flea) and fathead minnows, <br />between the control and any effluent concentration. Should <br />acute tonicity be detected in any effluent concentration less <br />than or equal to the Instream Waste Concentration (IWC) or <br />should a species mortality in any dilution of effluent <br />(including 100% effluent) exceed 50%, the permittee is required <br />to conduct a series of timely tests to identify and ultimately <br />eliminate or treat the toxicant. The IWC is determined using <br />the following equation: <br />IWC = [Facility Flow (FF)/(Stream Low Flow (annual) + FF)j X 100% <br />Using the facility design flow of 0.5 MGD and the acute stream <br />low flow (1E3) of 0 MGD, the IWC for the permittee is therefore <br />100%, which represents a wastewater concentration of 100% <br />effluent to 0% dilution water. <br />c) Chronic $iomonitoring: The determination as to whether or not <br />an individual facility must conduct chronic WET testing is <br />dependent upon the ratio of the chronic stream low flow (30E3) <br />to the design flow of the wastewater treatment plant, as well as <br />the receiving waters classifications. Since the receiving <br />waters are not classified as Aquatic Life Class 1, or Class 2 <br />with inorganic and metal numeric standards, chronic WET testing <br />is not required at this time. <br />d) Waiver of Requirements: Industrial facilities having simple <br />effluents of consistent quality, which can be totally <br />characterized chemically, may request a full or partial waiver <br />of WET testing for the remainder of the life of the permit, <br />subject to Division approval, if the first four quarters of <br />testing show no pattern of toxicity. <br />The permittee should read the WET testing sections of Part I.A. and <br />I.B. of the permit carefully, and should note that the test methods <br />for the toxicity tests are described in detail in the Division <br />guidance document, Guidelines for Conducting Whole Effluent <br />Toxicity Tests. This document should be read thoroughly prior to <br />commencing [he required WET testing, to ensure that the permittee <br />is aware of the various test conditions that could affect the test <br />results (e.g., sample holding time). <br />