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Enviro-Chem Analytical, Inc. Acute Toxicity Testing <br />685 West Gunnison Avenue, Suite 108 West Elk Mine -Jan. 2007 <br />Grand Junction, CO 81505 Outfall 017 <br />2 Species <br />Table Of Contents <br />Study Summary ....................................................................................1 <br />Introduction ............................................................................................2 <br />Methods .................................................................................................2 <br />Sample Collection ........................................... ............................2 <br />Sample Preparation and Usage ........................ ..........................3 <br />Test Performance ........................................... .............................3 <br />Test Acceptability ............................................ .............................4 <br />Statistical Analyses ......................................... .............................4 <br />Results .....................................................................................................5 <br />Effluent Chemistry ResuRs ............................................................5 <br />Effluent Toxicity Results for Fathead Minnows ...............................5 <br />Effluent Toxicity Results for Daphnia magna .................................5 <br />References ................................................................................................6 <br />Table 1. Arrival Chemistry Values for Final Effluent Sample from West Elk <br />Mine Somerset, Colorado <br />Table 2. Initial Chemistry Ranges for Fathead Minnow <br />96-hour Acute Toxicity Tests Conducted with T <br />West Elk Mine Effluent -Test Dates Jan. 16-20, 2007 <br />Table 3. Final Chemistry Ranges for Fathead Minnow and Daphnia magna <br />Toxicity Tests Conducted with West Elk Mine Effluent -Test Dates <br />Jan. 16-20, 2007 <br />Table 4. Percentage Survival Larval Fathead Minnows After 96-hour <br />Exposure to West Elk Mine Effluent -Test Dates Jan. 16-20, 2007 <br />