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<br /> <br />P=.RT I <br />Page le of 19 <br />Permit No. CO-0038024 <br />.-.. TERMS AND CONDITIONS <br />7. Acute Toxicity Level - Whole Effluent Toxicity (WET) Testing (004, 023, 024, <br />026 and 027) <br />Beginning immediately, there shall be no acute toxicity demonstrated in any <br />effluent concentration less than or equal to the instieam waste concentration <br />(IWC) of 100X. <br />Acute toxicity occurs when there is a statistically significant difference (P a <br />.OS, number of test organisms per dilution greater than or equal to 20) in the <br />mortality observed for either species between the control and any effluent <br />concentration. The statistical methods used shall be those in Short-Term <br />Methods For Estimatine The Chronic Toxicity Of Effluent and Receivine Waters To <br />Freshwater Or~ar.isms (EPA/600/4-85/014). <br />Also, beginning immediately, in no case shall mortality in any dilution of <br />effluent (including 100% effluent) exceed X07.. Mortality in the control must <br />simultaneously be 107. or less for the results co be considered valid. If the <br />mortality in the control exceeds 10%, the acute toxicity test must be repeated. <br />Amended Effective <br />