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~~ <br />Narrative and Discussion <br />• PERMIT AND SAMPLE INFORMATION <br />NPDES permit #: <br />Type of sample: <br />Date and time of sample(s): <br />Date and time of test start: <br />Dilution water: <br />Receiving temperature(s): <br />CO-0000213 <br />Effluent Grab <br />11/14/05 1030 <br />11/15/051125 <br />Reconstituted Laboratory Water <br />3.0 ° C <br />• TEST ORGANISMS <br />Fathead minnow <br />Daphnia magna <br />Age: 10 days <br />Age: <24 hrs <br />• PROCEDURE <br />Acute, 48 and 96-hour bioassays as outlined in the most recent protocols specified by the Water <br />Quality Control Division of the Colorado Department of Health (1998). <br />• DISCUSSION <br />According to the Western Fuels -Colorado discharge permit (CO-0000213), an acute WET test is <br />failed whenever the LC5°, which represents an estimate of the effluent concentration which is <br />lethal to 50% of the test organisms in the time period prescribed by the test, is found to be less <br />than or equal to 100% effluent. <br />By this definition, the results of this bioassay indicate thal effluent from ouffatl 007 is not acutely <br />toxic to fathead minnow and Daphnia magna survival. Therefore, outfall 007 is in compliance <br />with the acute WET portion of its Colorado State Industrial General Minimal Discharge Permit for <br />the. fourth quarter of 2005. <br />• DATE OF REPORT <br />November 23, 2005 <br />Report p/rep,~aredQby:J Heath Smith <br />d~1 t1-~3~05 <br />Biomonitoring Supervisor Date <br />Repo~rt/revi4ewed by: Heath Smith <br />I~+N~`r 1I-23~ <br />Biomonitoring Supervisor Date <br />4 <br />F52G <br />