HYDRO22447

• Permit No: C1981008
• IBM Index Class Name: Hydrology
• Doc Date: 1/30/2006
• Doc Name: 4th Quarter 2005 DMRs ‎(CO-000213)‎
• From: Western Fuels-Colorado, LLC
• To: WQCD
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

~GS~
Table of Contents
Abstract
Methods
Results
Narrative & Discussion
Table 1 -Colo. Test Conditions
Page 2 Table 2 -Methods and Equipment
Page 2 References
Page 3 Discharge Monitoring Report Form
Page 4 Reference Toxicity Data Chart(s)
Page 5 Supplement Forms'
Page 5
Page 5
Pg. 6-7
Pg. 8-9
Pg. 10
Abstract
SGS Minerals Services Division conducted a two species acute bioassay on Western Fuels New Horizon
Mine effluent discharge. This discharge has been designated as ouffall 007. The purpose of this
bioassay was to determine if the facility complies with the acute WET portion of the Colorado State
Industrial General Minimal Discharge Permit. The test organisms utilized were the fathead minnow
(Pimephales promelas) and cladoceran (Daphnia magna). Test results indicate that the effluent was not
acutely toxic to fathead minnow and Daphnia magna survival. Therefore, outfall 007 is in compliance
with the acute WET portion of its Colorado State Discharge Permit for the fourth quarter of 2005.
Methods
A Iwo species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine,
outfall 007. An effluent grab sample was collected on November 14, 2005, stored on ice, and delivered
to SGS Minerals Services Division on the next day. The efFluent sample was received at a temperature of
3.0° C and assigned sample tracking number 072-16623-001. The bioassay was initiated approximately
25 hours after sample collection.
Testing was conducted in accordance with the most recent protocols specified by the Water Quality
Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics
of the effluent and dilutions were measured by the methods and equipment outlined in Table 2.
The tests exposed the cladocerans, Daphnia magna, and fathead minnows, Pimephales promelas, to
five concentrations of efFluent, 100%, 75%, 50%, 25°!°, and 12.5%, prepared using reconstituted
laboratory water at 85-185 mg/L CaCO3. Control replicates were exposed to 100% laboratory water.
Statistically, the Probit and Binomial methods determined the LCSO sZ. Significant differences in mortality
were analyzed using the Steel's Many-One Rank test.
Supplemental forms include statistical analysis and chain-of-custody forms
z LCs° - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test
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