HYDRO22447

• Permit No: C1981008
• IBM Index Class Name: Hydrology
• Doc Date: 1/30/2006
• Doc Name: 4th Quarter 2005 DMRs ‎(CO-000213)‎
• From: Western Fuels-Colorado, LLC
• To: WQCD
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

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Table of Contents
Abstract Page 2
Methods Page 2
Results Page 3
Narrative & Discussion Page 4
Table 1 -Colo. Test Conditions Page 5
Abstract
Table 2 -Methods and Equipment
References
Discharge Monitoring Report Form
Reference Toxicity Data Chart(s)
Supplement Forms'
Page 5
Page 5
Pg. 6-7
Pg. 8-9
Pg. 10
SGS Minerals Services Division conducted a two species acute bioassay on Western Fuels New Horizon
Mine effluent discharge. This discharge has been designated as outfall 008. The purpose of this
bioassay was to determine if the facility complies with the acute WET portion of the Colorado State
Industrial General Minimal Discharge Permit. The test organisms utilized were the fathead minnow
(Pimephales promelas) and cladoceran (Ceriodaphnia dubia). Test results indicate that the effluent was
not acutely toxic to fathead minnow and Ceriodaphnia dubia survival. Therefore, ouffall 008 is in
compliance with the acute WET portion of its Colorado State Discharge Permit for the fourth quarter of
2005.
Methods
A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine,
outfall 008. An effluent grab sample was collected on December 13, 2005, stored on ice, and delivered
to SGS Minerals Services Division on the next day. The effluent sample was received at a temperature of
4.0° C and assigned sample tracking number 072-17072-001. The bioassay was initiated approximately
28 hours after sample collection.
Testing was conducted in accordance with the most recent protocols specified by the Water Quality
Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics
of the effluent and dilutions were measured by the methods and equipment outlined in Table 2.
The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promelas, to
five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted
laboratory water at 85 mg/L CaCOa. Control replicates were exposed to 100% laboratory water.
Statistically, the Binomial method determined the LCD sZ. Significant differences in mortality were
analyzed using the Steel's Many-One Rank test.
~ Supplemental forms include statistical analysis and chain-of-custody forms
2 LCD - an estimate of the concentration lethal to 50°!° of the organisms in the time prescribed by the test
2
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