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Client: Mountain Coal Company CO-0038766 SCG Project No.: 305140 <br />Site: East Sly Gulch <br />Acute Toxicity Test Summary <br />Test: • . 48-hour static renewal using Daphnia magna <br />• 96-hour static renewal using Fathead minnows (Pimephales <br />Client: Mountain Coal <br />Test Procedure Followed: EPA-821-R-02-012 <br />Sample Description: 305140 <br />Time of Sample Date of Sample Time of Sample Date of Sample <br />Collection Collection Receipt Receipt <br />1330 06-06-2005 1000 06-07-2005 <br />~~~~~ <br />~~~ <br />~.. w x <br /> <br />Daphntaltnagn ~ a <br /> <br />~ ~F¢~ +n .t <br />Test Initiation Time 1430 1445 <br />Test Initiation Date 06-07-2005 06-07-2005 <br />Test Completion Time 1340 1345 <br />Test Completion Date 06-09-2005 06-11-2005 <br />Dilution Water: • Moderately hard laboratory reconstituted water <br />Test Organism Source: • Daphnia magna <br />The SeaCrest Group <br />• Fathead minnows <br />The SeaCrest Group <br />Reference Toxicant: • Sodium Chloride <br />The eaCrest Group 3 <br />