HYDRO22341

• Permit No: C1980007
• IBM Index Class Name: Hydrology
• Doc Date: 8/1/2005
• Doc Name: Results of Acute Biomonitoring Tests East Sly Gulch Site
• From: The Sea Crest Group
• To: Mountain Coal Company, LLC
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

The SeaCrest Group Fathead ^9innow Acute Benchsheet Form #: 104a
Louisville, CO Effective: Juty 2002
Permitte~
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Site: (]J ~i~
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Lab#: ~b~l~'
ryyC^/°: ~ Template #: -x Sample Date: OL oG U S Dilution HiO: Fj U ~"'_ 02c~ ~~4
~
Species Info: ~ ~
LM 0 [.°G Z~S
i~ Test Started: 6 b b . D ~ ) tlllfZ Test Ended:
C'.>(~ t t~~- j~3y~'~~
Test Conditions: .
Water Quality Readings
FHM Vitality
Conc Readin 0 96 Re 0 2 4 48 72 9f
DO `~ ~ -'7-2 "l.•> (,,.ci ~
~Z iv-3 ~. t G ~ 1 10 I ~ -
,,,( Tem °C a, .E t°t 2i S ''`
. °. i W.t Z,. .S I z. ( L.'L 2 10 10 ~~ t:)
}(~ H Q.',Z ° I ° ^~ 0 _i ..7::' I ° . t `* %5 3 10 ~~' / J .7 GJ
/ Cond 30` 4 10 I. •- J 7 I ~

Tem °C 2~•`7 ~ - 1 l z. I c.. 7-L,.~
4 2 10 t=' ,o W o
9 H y ".3 ~ Z c I ;
. 3 10 t~ ~ I~ iv
Cond 4 10 1 .~ (~ t t->
DO `t . i I ~/. Z Co. S . y ' '"7 , .~ 1 10 ~ = t D J
Tem °C q, a. ~ °L- ZI - - c.. 7 l ~ -. 2„~{ ! , J .. t 2 10 l o Lo t o t

Cond 26 4 10 u O L;. ~
DO
Tem °C
'/ 0.`}
~ i`7."
J I2..~-7 6 ~ '3
z"-? I !y.
W G.°t I' .2
~, .yo, 0 4 1
2 10
10
=~
~ ._.
. jo
~
~~ H ~ d i "'.5 ov. .,.i LL G.C7 °~.r ~ 3 10 ~ 1~ V .~
Cond 60 4 10 17 ~

Tem °C `,~b. f3 4- LI : , _ 2u.'3 1
V _ , V . i°'t sj _, 2 10 J o J t ~
U H f7 •& ~ ~ JI ~~ 3 t0 J w t b t~
Cond a ;; - 4 10 ' J (~ \ ~ t

iu~ Tem °C `~-D ~~- ~L- \ I'Zay 20.2 i ?f..- ~- .-o ' tG7-$ m_2 2 10 IJ .J

Cond 4 10 o J J .J
DO u '_ 1 10
Tem °C ' 2 10
H 4a 3 1D
Cond 4 10
m la s
' eo ~ ;~ ~~ ~;~ r-;~ .~.~~>+*' ,inn
EH ecg Recon Recon Re- n Rec' Recon Recon Recon
Hard X13 Chlor Lo.oj Loci
AIk 50 Amm 1 Li. ~ u.o
Amm 2
1. Exposure Chamber
Total Capacity:
Test Solution Surface Area:
2. Feeding Schedule
Not Fed:
Fed Irregularly:
(describe):
3. Aerafion
None:
Before Use:
Slow:
Moderate:
Vigorous:
From:
To:
270 ml Test Solution Volume: 200 ml
cmZ Water Depth (constant): cm
(cyclic): cm
Fed Daily:
X Food Used: < 24 hr anemia
0&46hrs
( minutes @ -100 bubbles/min)
(-30 bubbles or mUmin)
(-60 bubbles or mL/min)
(-100 bubbles or mUmin)
AM/PM (Date)
AM/PM (Date)
4. Screened Animal Enclosures
Not Used: X Used: cm diameter
5. Condition/appearance of surviving organisms at end of test (i.e., alive but immobile, loss of orientation, erratic movement, etc.}:
~(~Jr. H t/~tilc.