HYDRO22341

• Permit No: C1980007
• IBM Index Class Name: Hydrology
• Doc Date: 8/1/2005
• Doc Name: Results of Acute Biomonitoring Tests East Sly Gulch Site
• From: The Sea Crest Group
• To: Mountain Coal Company, LLC
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

The SeaCrest Group Daphnid Acute Benchsheet Form #: 102a
Louisville, CO Effective: February 2002
Permitfee: ~'}, ~r~A>/w. EIk1`{ine ste:017 ~~~ ins+.~~Gulcl-,Lab#:305-Iy-~
IWC %: ~ Template#: = Sample Date: C~fJ(n(`~ri Dilution Water: M~-}
Age 8 Source: Q(n070`i - i~ j(~ Test Started: Q(p O~b._ - jy"30 Test Ended: O(o O f O S - /3 /v
Test Conditions:
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1. Exposure Chamber
Total Capacity: 30 ml Test Solution Volume: 15 ml
Test Solution Surface Area: cm` Water Depth (constant): cm
(cyclic): cm
2. Feeding Schedule
Not Fed: Fed Daily:
Fed Irregularly: X Food Used: YCT, Algae
(describe): fed 1-2 hours before test
3. Aeration
None:
Before Use: ~_minutes @ -100 bubbles/min)
4. Screened Animal Enclosures
Not Used: X Used: cm diameter
5. Condition/appearance of surviving organisms at end of test (i.e., alive but immobile, loss of
orientation, erratic movement, etc.): a C 72 V i`L. %)N'L1 bN o 6/ c,~ ~ 5 v 2 V f V W p r.2 V S ~ N
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6. Comments: