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• <br />B. MONITORING REQUIREMENTS <br />Part I <br />Page ld of 19 <br />Permit No. CO-0042161 <br />3. Frequency and Sample Type - Acute WET Testing (Cont.) <br />The permittee shall conduct each acute WET test in general accordance <br />with methods described in the Division guidance document entitled <br />Guidelines for Conducting Whole Effluent Toxicity Tests. The permittee <br />shall conduct an acute 48-hour WET test using Ceriodaphnia sp., and an <br />acute 96-hour WET test using fathead minnows. Acute tests will be <br />replacement static tests of a single effluent grab sample. <br />4. Accelerated Testing <br />If an individual toxicity test shows toxicity at concentrations less <br />than or equal to the Instream Waste Concentration (IWC) of 62X or if SOX <br />species mortality in any dilution is detected in any acute WET test <br />conducted in accordance with this permit, the permittee shall notify the <br />Division verbally within 24 hours and in writing within 5 days of <br />becoming aware of the toxicity. Within 7 days of becoming aware of the <br />toxicity, the permittee shall then begin accelerated testing of the <br />• discharge with whichever species has proven (through the first instance <br />of toxicity) to be the most sensitive. The permittee will continue such <br />testing with one species, once every week until directed by the Division <br />to do otherwise, but in no case for more than five representative tests. <br />5. Preliminary Toxicity Investigation <br />If directed by the Division, the permittee shall perform a preliminary <br />toxicity investigation. The permittee will have 15 working days from <br />the date of Division notification to complete a preliminary toxicity <br />investigation and submit the results to the Division, in writing. The <br />preliminary toxicity investigation may include, but is not limited to: <br />additional chemical and biological monitoring, examination of <br />pretreatment program records, examination of discharge monitoring <br />reports, evaluation of treatment processes and chemical use, inspection <br />of material storage and transfer areas to determine if a spill may have <br />occurred, and similar procedures. <br />If the preliminary toxicity investigation identifies a probable toxicant <br />and/or a probable source of toxicity, the permittee shall submit, as <br />part of its final report results, written notification to that effect to <br />the Division. Within 30 days of completing the preliminary toxicity <br />investigation, the permittee shall submit for the Division's approval a <br />control program to control effluent toxicity, and shall proceed to <br />implement such plan withi^ 7 days following approval by the Division. <br />• Revised S/2 /89 <br />Doc 1003M <br />