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Western Fuels 008 CD Acute Sampled 9/5/2006 <br />Program run on 09-12-2006 at 11:37:48 <br />RESULTS CALCULATED USING THE BINOMINAL METHOD <br />CONC. NUMBER NUMBER PERCENT BINOMIAL <br /> EXPOSED DEAD DEAD PROB. (PERCENT) <br />100 20 8 40.00 25.1722 <br />75 20 3 15.00 0.1288 <br />50 20 4 20.00 0.5909 <br />25 20 0 0.00 LESS THAN 0.001 <br />12.5 20 5 25.00 2.0695 <br />AT A CONFIDENCE LEVEL OF 95 PERCENT, THE BINOMIAL TEST <br />SHOWS THAT THE LC50 IS ABOVE 75 <br />THE USEFULNESS OF ANY LC50 CALCULATED FROM THIS SET OF DATA <br />IS QUESTIONABLE BECAUSE A CONCENTRATION-EFFECT RELATIONSHIP <br />HAS NOT BEEN DEMONSTRATED OVER A REASONABLE RANGE (e.g. <br /><37 TO>63 OF PERCENT DEAD <br />NEITHER THE APPROXIMATE LC50 CALCULATION NOR THE MOVING <br />AVERAGE METHOD CAN BE USED WITH THIS SET OF DATA. <br />EITHER THE HIGHEST CONCENTRATION KILLED LESS THAN 50 <br />PERCENT OR THE LOWEST KILLED MORE THAN 50 PERCENT. <br />IF THE PROBIT SLOPE IS NEGATIVE, ENTER THE DATA AGAIN <br />USING THE NUMBER ALIVE INSTEAD OF THE NUMBER DEAD. <br />RESULTS CALCULATED USING THE PROBIT METHOD <br />GOODNESS OF FIT <br />ITERATIONS G H CHI-SQUARE PROBABILITY <br />4 23.302 3.172 9.516 0.023 <br />BECAUSE THE PROBABILITY IS LESS THAN 0.05, RESULTS <br />CALCULATED USING THE PROBIT METHOD PROBABLY SHOULD NOT <br />BE USED. <br />SLOPE _ .5319583 <br />95 PERCENT CONFIDENCE LIMITS = -2.0359 AND 3.09981'7 <br />LC50 = 1656.05 <br />95 PERCENT CONFIDENCE LIMITS = 47.76656 AND + INFINITY <br />LC1 = 7.014335E-02 <br />95 PERCENT CONFIDENCE LIMITS = 0 AND 18.35962 <br />COMPARE RESULTS WITH ORIGINAL DATA TO SEE IF THEY ARE <br />REASONABLE. <br /> <br />