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'~~ <br />Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplement Forms' Pg. 10 <br />Abstract <br />SGS North America Inc. conducted a two species acute bioassay on Western Fuels New Horizon Mine <br />effluent discharge. This discharge has been designated as outfall 008. The purpose of this bioassay <br />was to determine if the facility complies with the acute WET portion of the Colorado State Industrial <br />General Minimal Discharge Permit. The test organisms utilized were the fathead minnow (Pimephales <br />• promelas) and cladoceran (Ceriodaphnia dubia). Test results indicate that the effluent was not <br />acutely toxic to fathead minnow and Ceriodaphnia dubia survival. Therefore, outfall 008 is in <br />compliance with the acute WET portion of its Colorado State Discharge Permit for the second <br />quarter of 2006. <br />Methods <br />A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine, <br />ouffall 008. An effluent grab sample was collected on May 25, 2006, stored on ice, and delivered to SGS <br />North America Inc. on the next day. The effluent sample was received at a temperature of 3° C and <br />assigned sample tracking number 072-19777-001. The bioassay was initiated approximately 26 hours <br />after sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water, Control replicates were exposed to 100% laboratory water. <br />Statistically, the Binomial method determined the LCso's2. Significant differences in mortality were <br />analyzed using the Steel's Many-One Rank test. <br /> <br />' Supplemental forms include statistical analysis and chainof-custody forms <br />Z LCso - an estimate of the concentration lethal to 50% of the organisms in the time prescribed by the test <br />2 <br />F-526 <br />