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METHODS <br />A single species acute bioassay was conducted with wastewater taken <br />from outfall 002 at Trapper Mining's Craig Mine. A grab sample was <br />collected on July 12, 1994, delivered to Commercial Testing and <br />Engineering (CT&E) on July 13, 1994, and assigned CT&E number 72- <br />281057. The tests were initiated approximately 30 hours after <br />sample collection. <br />Testing was conducted in accordance with the most recent protocols <br />specified by the Water Quality Control Division of the Colorado <br />Department of Health (1992), outlined in Table 1. <br />The chemical characteristics of the effluent that were measured <br />included pH, ammonia, residual chlorine, alkalinity, hardness, <br />dissolved oxygen and conductivity. <br />The pH was measured using a Fisher Scientific model 925 pH meter <br />(EPA method 150.1) and ammonia was determined with an Orion 901 <br />ionalyzer (EPA method 350.2). Total residual chlorine was measured <br />using a LaMotte model STC-C1 colorimeter (EPA accepted). <br />Alkalinity and hardness were determined by titrimetric methods <br />specified by the American Public Health Association, 1989, (EPA <br />methods 310.1 and 130.2), dissolved oxygen was measured with a YSI <br />model 54A oxygen meter (EPA method 360.1) and conductivity was <br />determined with a YSI model 31 conductivity meter (EPA method <br />120.1). <br />The tests exposed cladocerans, Ceriodabhnia dubia, to five <br />concentrations of effluent, 100$, 75$, 50$, 25$ and 12.5$, prepared <br />using reconstituted laboratory water at 116 mg/L CaC03. Control <br />replicates were exposed to 100$ reconstituted laboratory water. <br />-2- <br />COMMER CIALTESTING & ENGINEERING CO. <br />Original Copy Waiermarketl <br />For YOar Proleclion <br />F166 <br />LTA <br />