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W.E'. T. Inc. <br />Water & Enviranmenta! Tutinr Inc 399 West 1200 North. 8 Orem. Ulah 84057 /801)135-9741 Fax/801)135-9560 <br />Acute Whole Effluent Toxicity Report <br />Ceriodaphnia <br />DATE: July 7, 2000 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (AnimaUAgeJ: Ceriodaphnia Q4 hours. SAMPLE (Date/TypeJ: 620/00 Grab. <br />DATE/I'IME TEST BEGAN: 6/21/00 11:35 a.m. DATE/1'IME TEST COMPLETED: 6!23/00 12:35 p.m. <br />TEST CONDITIONS <br />Ceriodaphnia dubia neonates were exposed to diluted EfFluent as specified by the permit. At the end ofthe test period Survival <br />was measured to determine if Acute Toxicity was present in the sample. <br />Animal Age et. Test Start <br />Number ofOrganisms/Dilution Volume/Replicates <br />Food <br />Aeration <br />I)issolved.Oxygeo .. <br />Water Replacement <br />Temperature. ..:.:. <br />Pboto Period <br />PH. .. .. .:..... <br />Dilution Water <br />;Receiving Water .. <br /><24 hours... ... - :.P .:;: :. . <br />5 organisms/15 mI/4 replicates. <br />Fed YTC and Algae before sfaning test. <br />None required. <br />Measured daily old/new! <br />Renewed daily. <br />20 +. l degrees C:. . <br />16 hours light 8 hours dark. <br />Measuredinitially.and at 24 hottrs: <br />Reconstituted water (+/- I S% hardness of the <br />receiving water). <br />None.was supplied. <br />SUMMARY <br />Results: X . Pass Fail <br />There was NO significant effect on survival. <br />Enclosed are data sheets and statistical reports. <br />Sincere) <br />Rawlings <br />Water & Envvonmental Testing, Inc. <br />Enclosure <br />