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W. ~' . T. Inc. <br />Wager & Environments/ TestinY Inc. 399 West 1100 North. B Orem. Utah 84057 (8011135-9741 Fax/801 J235-9560 <br />Acute Whole Effluent Toxicity Report <br />Fathead Minnow <br />DATE: July 7, 2000 <br />PERMITTEE NAME: Western Fuels Co. SITE ID: New Horizon Mine OUTFACE NO: 007 <br />TEST (AnimaUAge): Fathead Minnow, 7 days. SAMPLE (Date/Type): 6!20/00 Grab. <br />DATE/I'IME TEST BEGAN: 6/21/00 1 I :45 a.m. DATE/I'IME TEST COMPLETED: 6!25/00 2:00 p.m. <br />TEST CONDITIONS <br />Fathead Minnow larvae were exposed to diluted effluent as specified by the permit. At the end of the test period Survival was <br />measured to determine if Acute Toxicity was present in the sample. <br />Animal Age at.Test Start. Fathead Minnow, 7 days; is <br />Number of Organisms/Dilution Volume/Replicates 10 organisms/I50 mIJ2 replicates <br /> i <br />:Fed before starting test and'af 48 hours, newly ha(ched <br />Food brine.shrimp. i ~ :. .. . <br />~ Aeration None required. <br />Dissolved Oxygen . Measured daily old/new. <br />Water Replacement Renewed daily. <br />Temperature . .. 20.+ I degrees C. .. . ; ..; 1 :.... . <br />Photo Period 16 hours light 8 hours dazk. <br />pH Measured initially and at 24'hours.. <br />Dilution Water Reconstituted water (+/- I S% hardness of the <br /> receiving water). <br />Receiving Water <br />r.. <br />Nope was supplied. <br />SUMMARY <br />Results: X : Pass Fail <br />There was NO significant effect on stavival. <br />Enclosed are data sheets and statistical reports. <br />Sincerely, <br />Rawlings <br />Water & Envvonmental Testing, Inc. <br />`-'` Enclosure <br />