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Table of Contents <br />Abstract Page 2 Table 2 -Methods and Equipment Page 5 <br />Methods Page 2 References Page 5 <br />Results Page 3 Discharge Monitoring Report Form Pg. 6-7 <br />Narrative & Discussion Page 4 Reference Toxicity Data Chart(s) Pg. 8-9 <br />Table 1 -Colo. Test Conditions Page 5 Supplement Forms' Pg. 10 <br />Abstract <br />SGS North America Inc. conducted a two speces acute bioassay on Westem Fuels New Horizon Mine <br />effluent discharge. This discharge has been designated as outtall 008. The purpose of this bioassay <br />was to determine if the fadlity complies with the acute WET portion of the Colorado State Industrial <br />General Minimal Discharge Permit. The test organisms utilized were the fathead minnow (Pimephales <br />promelas) and dadoceran (Ceriodaphnia dubia). Test results indicate that the effluent was not <br />acutely toxic to fathead minnow and Ceriodaphnia dubia survival. Therefore, outfall 008 is in <br />compliance with the acute WET portion of its Colorado State Discharge Permit for the third <br />quarter of 2007. <br />Methods <br />A two species acute bioassay was conducted with effluent from the Western Fuels New Horizon Mine, <br />outtall 008. An effluent grab sample was collected onJuly 09, 2007, stored on ice, and delivered to SGS <br />North America Inc. on the next day. The effluent sample was received at a temperature of 8.9 °C and <br />assigned sample tracking number TA7-GO-P023-001. The bioassay was initiated less than 24 hours <br />after sample collection. <br />Testing was conducted in accordance with the most recent protocols specified by the Water Quality <br />Control Division of the Colorado Department of Health (1998) outlined in Table 1. Basic characteristics <br />of the effluent and dilutions were measured by the methods and equipment outlined in Table 2. <br />The tests exposed the cladocerans, Ceriodaphnia dubia, and fathead minnows, Pimephales promelas, to <br />five concentrations of effluent, 100%, 75%, 50%, 25%, and 12.5%, prepared using reconstituted <br />laboratory water. Control replicates were exposed to 100% laboratory water. <br />Statistically, the Binomial method determined the LC~'sz. Significant differences in mortality were <br />analyzed using the Dunnett's test. <br />' Supplemental forms include statistical analysis and chain-ofcustody forms <br />z LCw - an estimate of the concentrafion lethal to 50% of the organisms in the time prescribed by the test <br />2 <br />