HYDRO20023

• Permit No: C1980007
• IBM Index Class Name: Hydrology
• Doc Date: 8/12/2005
• Doc Name: Reports for Acute Biomonitoring Tests
• From: The Sea Crest Group
• To: Mountain Coal Company, LLC
• Permit Index Doc Type: DMR’s
• Media Type: D
• Archive: No

The SeaCrest Group Fathead R~innow Acute Benchsheet Form #: 1D4a
Louisville, CO Effectivz: July 2D02
Permiftee: ~~'\r L-=,'~` Site: (71T [-~F E~r~~~ .~~.SS, Lab#: ~-~p /~)'
IWC%; ~G Template #: ( Sample Dafe: (j O(~Cj Dilution HZO: p p c G. 't
Species Info: µ-,%efv'~^ (~(~t~(US Test Started: V(Gp7 r: ~' 1. Gv Test Ended: _ (7o tips - lV '
Test Conditions:
Water Quality Readings
FHM Vitality
Conc Readin 0 96 Re 0 2 4 48 72 91
DO y- " - o i _q 7~, ~ i J 7.-s i .-Z- , ~- 1 10 ~ i K
r'~ Tem °C 2~. tci.'Z,I i.~. '1o- II°I,Z :;. I o.' 2 10 ~ ~ (a t.~ 1=
~/ H ;.i vl 4I o I d. ~; ;~~ '(.. 3 10 I~ fQ to to
Cond 4 10 (~ u ~ ~
DO c° 4 9 ° ~-' ' t 7• ~ 1 10 J p ~
Tem °C Z•.~i 'R- .~v v. I 1 .I t~.~ 170 t~ ' Z 10 ~,~ v r ~ (J
.G H ~ Z 1 ~.Z 2 1 ~.~1 ~.") ~.\ 3 10 t~
Cond z 4 10 ~~ ~ -
DO .0 7-u i-7--' -7-~' '~ 1 10 O i to
~ T
m °C q 4.
L
J Z
~ ? I 0
IZ. H J I
, . L: ~ C ~1 .
L (D 3 10 /~ J to J
Cond 4 10 t~ J l~ S

Tem °C .,,: w° «-Z 12°.5 .2 .Y.`3 °w _ .I Z+>~ ~. 2 10 ;~ lu t~ t~
~~ H o ~ ;. I ;,.5 ~. °,.6 ~ ~ ~ ;.v 3 10 r.o h
Cond "1L 4 10 _ 't"~
DO 'Z. i-7.Z @ ~ U.S ~ Z.L 1 10 ~o u 1~ t%
~U Tem °C ft.? 12J> o• I ,5 ° IZ- Z°•1 2 10 t-~ i~ t~ t°

Cond 'L~o 4 10 IJ r~ ~o l
J
I,°nl Tem °C ° °• t~. I r`~.~ ~ , : I .9 ' 7.r.`f i Z,,. ~ . 2 10 U I' i J
6/l/t/ H ~.:f ° eo I ~. s~ U I g c ~ c Si ~ 3 10 ~ t ~ )
Cond R 4 10 iJ w t ,,~
DO 1 10
.l Tem °C 2 10
H I 3 10

m Cond
is s
EH

ecg

Recon Recon

Recon Rec'

e n 4
:O ks"t,~#.
Recon 10
€fiv?+itN+
Recon
xft2s ";. 2,s~:~7.i "`ifs
Hard ~}~ Chlor L o. of ~.o 01
Alk -' io0 Amm 1 GLO fl,u
Amm 2 --
1. Exposure Chamber
Total Capacity:
Test Solution Surface Area:
2. Feeding Schedule
Not Fed:
Fed Irregularly:
(describe):
3. Aeration
None:
Before Use:
Slow:
Moderate:
Vigorous:
From:
To:
4. Screened Animal Enclosures
Not Used:
5. Condition/appearance of surv
v~( JUL. `~ !~~\j
270 ml Test Solution Volume: 200 ml
cmZ Water Depth (constant): cm
(cyclic): cm
Fed Daily: _
X Food Used: < 24 hr artemia
0&48hrs
( minutes @ -100 bubbleslmin)
(-30 bubbles or mUmin)
(-60 bubbles or mUmin)
(-100 bubbles or mUmin)
AM/PM (Date)
AM/PM (Date)
X Used: cm diameter
organisms at end of test (i.e., alive but immobile, loss of orientation, erratic movement, etc.):
6. Comments: