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Technical Memo  Page 4 August 16, 2024 <br />Peabody – CDPS Permit Supplement <br />•Evaluation of selenium concentrations in water (potentially dissolved, dissolved, <br />and total fractions) <br />Collected on an ongoing basis instream and at outfalls by mine personnel <br />2.2 Summary of Selenium Study Results <br />The limited presence of water throughout many of these drainages, especially in the upper <br />reaches, adversely impacts the ability of these streams to support robust fish communities <br />except for select downstream locations on Cow Camp Creek and Bond Creek. Flow <br />conditions vary substantially throughout the year with some sites being partially or <br />completely frozen in winter, and only intermittently wetted during snowmelt runoff and <br />precipitation events. In addition, the overall habitat complexity, as determined by the number <br />and type of habitat units observed, is generally low at most sites. These conditions have been <br />well documented in past Use Attainability Analyses (UAAs) submitted during previous basin <br />hearing cycles, including GEI 2014 and 2017, and the selenium study updates provided in <br />2020, 2021, 2022, and 2023 (GEI 2014, 2017, and 2020, 2021, 2022, 2023). <br />Seasonal sampling has revealed marginal benthic macroinvertebrate populations and the <br />absence of fish in the upper reaches of Cow Camp and Bond creeks. Fish have been present <br />in almost all years in the lower reaches of Bond Creek Site G-BC-3, with only limited <br />populations present in some years in the lower reaches of Cow Camp Creek. The intermittent <br />flow and natural obstructions (e.g., plunge pools, beaver dams, wetlands, and seasonal <br />drying) limit the ability of Cow Camp Creek to support reproducing fish populations <br />consistently over time. Cow Camp and Bond creeks have flow durations that are restricted <br />predominantly to the snowmelt runoff period and often exhibit no flow by mid-to-late <br />summer. Therefore, the collection of fish tissues for analysis has focused on spring <br />monitoring for the lower extent of each drainage. <br />Selenium concentrations in the macroinvertebrate tissue collected is typically lower in the <br />upper drainage and then increases downstream, fish tissue results have also followed a <br />similar trend at the lower sample locations where populations persist, largely in response to <br />the influx of naturally occurring selenium from underlying geology. While Creek Chub and <br />White Sucker are both considered trophic level 3 species under the EPA’s TSDs, fish tissue <br />results indicate that Creek Chub generally have higher selenium tissue concentrations <br />compared to White Sucker at these sites. Creek Chub are also the more common species <br />found within these drainages, so the larger dataset available for Creek Chub may be <br />impacting this trend. <br />All sites discussed in this memo located on Cow Camp and Bond creeks, even if the reach is <br />fish-limited, show mean values by species for the comprehensive dataset below the EPA’s <br />whole-body fish tissue criterion of 8.5 milligrams per kilogram dry weight (mg/kg dw). The <br />dataset for egg/ovary samples is limited and should be interpreted cautiously. The 2016