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DocuSign Envelope ID: D07924E6-3D94-4398-A066-B683COBAB33E <br /> 4.4 Ceriodaphnia dubia Chronic Toxicity Test <br /> TEST: 7-day chronic with Ceriodaphnia dubia OPERATORS: AW, JMS, LAG, TE, JW <br /> Start: 1/19/21 11:40 <br /> End: 1/27/21 12:30 <br /> Test Substance: 0.5 g ethanol/g SO4 with/without lime pre-softening <br /> Client/Project: Elk Ridge Mining and Reclamation, LLC <br /> Table 12: Summary results of C. dubia chronic toxicity test. <br /> Treatment 0* 100(with 100 (without <br /> %Treated Effluent Lime Pre-softening) Lime Pre-softening) <br /> #alive/#exposed 6/10 0/10 0/10 <br /> %survival 60 0 0 <br /> Normalized repro/# 9.1 0.0 0.0 <br /> females exposed ±SD ± 12.4 ±0.0 ±0.0 <br /> Dissolved 02 range 7_0 6_5 6_5 <br /> (mg/L) 5.9 4.2 4.8 <br /> pH range(SU) 8_4 8_9 8_9 <br /> 8.0 8.5 8.6 <br /> Conductivity range 365 4380 4350 <br /> (pmho/cm) 328 3600 3200 <br /> Temperature range(°C) 24.9 24.8 25.0 <br /> 23.3 23.5 24.1 <br /> *Control performance criteria not met for survival and reproduction. <br /> Statistical Analysis not conducted due to control performance criteria not being met <br /> (invalid test) <br /> GEI Consultants,Inc. 14 February 2021 <br /> Ecological Division Whole Effluent Toxicity Test Report <br /> Stantec-Elk Ridge Mining and Reclamation,LLC <br />