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DocuSign Envelope ID: D07924E6-3D94-4398-A066-B683COBAB33E <br /> 2.0 Test Conditions <br /> 2.1 Ceriodaphnia dubia 7-day toxicity test to estimate chronic <br /> toxicity <br /> Method: EPA-821-R-02-013 -- Short-Term Method for Estimating Chronic Toxicity. . . <br /> Test Duration: until at least 60% of control organisms release 3 broods <br /> Sample Collection Procedure: grab <br /> Sample Collection Date: 11/4/20, 11/17/20, 1/18/21 <br /> Dilution water: moderately hard reconstituted water <br /> Age of organisms at start: <24 hours old <br /> Feeding: 0.1 mL YCT/0.1 mL Selenastrum daily <br /> End Point: mortality, normalized reproduction <br /> Start Date and Time: 0.5 g ethanol/g SO4 11/5/20 12:00 <br /> 1.0 g ethanol/g SO4 11/5/20 13:40 <br /> 0.25 g ethanol/g SO4 11/17/20 14:35 <br /> 0.5 g ethanol/g SO4 <br /> with/without <br /> lime pre-softening 1/19/21 11:40 <br /> End Date and Time: 0.5 g ethanol/g SO4 11/11/20 12:40 <br /> 1.0 g ethanol/g SO4 11/12/20 13:30 <br /> 0.25 g ethanol/g SO4 11/25/20 13:40 <br /> 0.5 g ethanol/g SO4 <br /> with/without <br /> lime pre-softening 1/27/21 12:30 <br /> Type of exposure chamber: 30 mL disposable plastic cup <br /> Volume of exposure chamber: 15 mL <br /> Number of animals exposed/chamber: 1 <br /> Number of replicates/treatment: 10 <br /> Test temperature: 0.5 g ethanol/g SO4 23.8°C ± 1.0°C <br /> 1.0 g ethanol/g SO4 23.6°C ± 1.6°C <br /> 0.25 g ethanol/g SO4 24.4°C ± 1.0°C <br /> 0.5 g ethanol/g SO4 <br /> with/without <br /> lime pre-softening 24.2°C ± 0.9°C <br /> EPA recommended range: 25.0°C± 1.0°C <br /> Standard toxicant used: NaCl <br /> GEI Consultants,Inc. 4 February 2021 <br /> Ecological Division Whole Effluent Toxicity Test Report <br /> Stantec-Elk Ridge Mining and Reclamation,LLC <br />