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Biological Assessment <br /> Federal Coal Lease Modification(COC-62920)and Federal Mine Permit(CO-0106A)Revision and Renewal <br /> campaign that replicated those populations across the landscape. Efforts to establish new <br /> populations were successful and populations are expected to meet recovery plan goals and <br /> become delisted. However, recent genetic work on museum and extant populations suggest that <br /> the true native greenback cutthroat trout occurs only in a single stream, Bear Creek, outside their <br /> historic range just west of Colorado Springs. Recent studies revealed that approximately 750 <br /> adult fish remain in Bear Creek and represent the last remaining individuals of the species. <br /> Aggressive recovery efforts have been implemented to replicate this population, with the first <br /> reintroduction in the wild occurring in August 2014. <br /> Life History <br /> Greenback cutthroat trout opportunistically feed on a wide range of prey organisms, but a large <br /> percentage of the diet is terrestrial insects. Greenbacks also feed on crustaceans (e.g., freshwater <br /> shrimp), aquatic insects, and small fish. Spawning typically occurs from late May to mid-July in <br /> higher elevations. Male cutthroats first spawn at age 2, and females mature a year later. Females <br /> build an egg pit in gravel generally 3 to 8 inches deep and 1 foot in diameter. A 10-inch female <br /> lays about 800 eggs. Larger fish of about 4 to 7 pounds lay up to 6,000 eggs. <br /> Habitat <br /> Greenback cutthroat trout occur in steep, cold-water mountain streams and lakes with clear, <br /> well-oxygenated water(Young 2009). <br /> Distribution and Status <br /> Until recently, delineations of subspecies of cutthroat trout in the southern Rocky Mountains <br /> were believed to follow geographic boundaries within several states, with greenback cutthroat <br /> trout on the east side of the Continental Divide and Colorado River cutthroat trout(O. C. <br /> pleuriticus) on the west side. Rio Grande cutthroat trout(O. c. virginalis) occur within the Rio <br /> Grande drainage; their range and genetic identity do not appear to be in question. <br /> Through the recent genetic (Metcalf et al. 2012) and meristic (Bestgen et al. 2013) studies that <br /> identified the native ranges of cutthroat in Colorado, we now know that greenbacks are native <br /> only to the headwaters of the South Platte River drainage in Colorado. Greenbacks were <br /> previously considered to be native also to the Arkansas River drainage (Behnke 1992). The <br /> original distribution of the greenback within the South Platte drainage is not precisely known due <br /> to its rapid decline in the 1800s. The loss of high-quality trout stream habitat through logging, <br /> livestock over-grazing, water diversions, mining, and municipal and industrial pollution is <br /> considered a contributing factor to the historical decline of the range of greenback. It is assumed <br /> that the original distribution included all mountain and foothill habitats of the drainage systems, <br /> including lower elevations than it occupies today (Behnke and Zarn 1976). The subspecies may <br /> have extended as far east as present day Greeley, Colorado, during the mid-1800s (WNTI 2007). <br /> July 2017 <br /> 23 <br />