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DocuSign Envelope ID:6FE5F752-9229-4969-B8B6-9154B88CD3C4 <br /> Client: Colowyo CO-0045161 SCG Project No.: 420469.E <br /> Site: 010A <br /> Chronic Toxicity Test Summary <br /> Test: • 7-day static renewal using Ceriodaphnia dubia <br /> • 7-day static renewal using fathead minnow (Pimephales <br /> promelas) <br /> Client: Colowyo Coal Company <br /> Test Procedure Followed: • Ceriodaphnia dubia: EPA/821/R-02-013. Method 1002.0 <br /> (2002) <br /> • fathead minnow: EPA/821/R-02-013. Method 1545.0 <br /> (2002) <br /> Sample Number: 420469.B <br /> Sample Time of Date of Time of Receipt Date of Receipt <br /> Collection Collection <br /> Effluent 1 0850 10-12-2020 0900 10-13-2020 <br /> Effluent 2 0935 10-14-2020 0931 10-15-2020 <br /> Effluent 3 0840 10-16-2020 0850 10-17-2020 <br /> Ceriodaphnia dubia fathead minnow <br /> Test Initiation Time 1345 1223 <br /> Test Initiation Date 10-13-2020 10-13-2020 <br /> Test Completion Time 1300 1150 <br /> Test Completion Date 10-19-2020 10-20-2020 <br /> Dilution Water: • Moderately hard laboratory reconstituted water <br /> Test Organism Source: • Ceriodaphnia dubia <br /> SeaCrest Group <br /> • fathead minnow <br /> SeaCrest Group <br /> Reference Toxicant: • Sodium Chloride <br /> SeaCrest Group 3 <br />